Back to browse

siRNA

Linked experiment: EXP001360

Linked peptide: PepFect 14 (PF14)

Linked paper: Targeted gene silencing in human embryonic stem cells using cell-penetrating peptide PepFect 14 (2019)

RNA overview

RNA Type

siRNA

Payload/target

Beta-2-microglobulin (B2M) siRNA (siB2M) for surface antigen knockdown

Target gene/transcript

gen

Sequence information

Sequence reported in source article

yes

RNA sequence

sense:ACGCCAAAAACAUAAAGAAAG; antisense:UUCUUUAUGUUUUUGGCGUCU

Sense strand

ACGCCAAAAACAUAAAGAAAG

Antisense strand

UUCUUUAUGUUUUUGGCGUCU

Chemical modifications

RNA modifications

Commercial Ambion Silencer Select siRNAs (OCT4 ID s10872; B2M ID s1854; Negative control #1). Fluorescent uptake tracer: Alexa Fluor 568–conjugated siRNA (Eurofins).; Alexa Fluor