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siRNA

Linked experiment: EXP001428

Linked peptide: Pep6

Linked paper: An Amphipathic Structure of a Dipropylglycine-Containing Helical Peptide with Sufficient Length Enables Safe and Effective Intracellular siRNA Delivery (2023)

RNA overview

RNA Type

siRNA

Payload/target

Firefly GL3 luciferase siRNA (Luc siRNA); sequences: sense 5'-CUUACGCUGAGUACUUCGAdTdT-3', antisense 5'-UCG AAG UAC UCA GCG UAA GdTdT-3' (dTdT overhangs). Control: Scrambled control siRNA (Scr siRNA); sequences: sense 5'-UUCUCCGAACGUGUCACGUdTdT-3', antisense 5'-ACGUGACACGUUCGGAGAAdTdT-3'.

Target gene/transcript

sense

Sequence information

Sequence reported in source article

yes

RNA sequence

sense:CUUACGCUGAGUACUUCGAdTdT; antisense:UCGAAGUACUCAGCGUAAGdTdT

Sense strand

CUUACGCUGAGUACUUCGAdTdT

Antisense strand

UCGAAGUACUCAGCGUAAGdTdT

Chemical modifications

RNA modifications

dTdT overhangs; Alexa488 label on antisense strand used for uptake experiments (separate assay).