pDNA

Linked experiment: EXP001434

Linked peptide: Penetratin (PEN)

Linked paper: Intracellular delivery of antiviral shRNA using penetratin-based complexes effectively inhibits respiratory syncytial virus replication and host cell apoptosis (2024)

RNA overview

RNA Type: pDNA
Payload/target: pGFP-V-RS (OriGene) plasmid encoding RSV F-gene shRNA (U6 promoter) and GFP marker (CMV promoter); shRNA targets RSV fusion (F) gene; shRNA sequence: GCAGTGCAGTCAGCAAAGGCTATCTTAGTTCAAGAGGCACTAAGATAGCCTTTGCTGACTGCACTGC (F-shRNA cassette; includes BamHI/HindIII sites)
Target gene/transcript: F-gene

Sequence information

Sequence reported in source article: yes
RNA sequence: sense:GCAGTGCAGTCAGCAAAGGCTATCTTAGT; antisense:ACTAAGATAGCCTTTGCTGACTGCACTGC
Sense strand: GCAGTGCAGTCAGCAAAGGCTATCTTAGT
Antisense strand: ACTAAGATAGCCTTTGCTGACTGCACTGC

Chemical modifications

RNA modifications: Plasmid DNA vector; GFP marker