siRNA

Linked experiment: EXP001618

Linked peptide: C6M3

Linked paper: Characterization and evaluation of a peptide-based siRNA delivery system in vitro (2017)

RNA overview

RNA Type: siRNA
Payload/target: GAPDH siRNA (Ambion Silencer™ GAPDH siRNA kit); Cy3-labeled GAPDH siRNA used for uptake assays
Target gene/transcript: GAPDH

Sequence information

Sequence reported in source article: yes
RNA sequence: sense:GACGUAAACGGCCACAAGUUC; antisense:ACUUGUGGCCGUUUACGUCGC
Sense strand: GACGUAAACGGCCACAAGUUC
Antisense strand: ACUUGUGGCCGUUUACGUCGC

Chemical modifications

RNA modifications: Cy3 label at 5′ end of sense strand for uptake/imaging; otherwise standard siRNA duplex