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siRNA

Linked experiment: EXP001735

Linked peptide: Penetratin — NPs-2 (HA 200 kDa, low density shell)

Linked paper: Fine Tuning of Core–Shell Structure of Hyaluronic Acid/Cell-Penetrating Peptides/siRNA Nanoparticles for Enhanced Gene Delivery to Macrophages in Antiatherosclerotic Therapy (2018)

RNA overview

RNA Type

siRNA

Payload/target

LOX-1 (lectin-like oxLDL receptor-1) siRNA

Target gene/transcript

not reported

Sequence information

Sequence reported in source article

yes

RNA sequence

sense:AGGACGGUUCUCCUUUGAUdTdT; antisense:dTdTUCCUGCCAAGAGGAAACUA

Sense strand

AGGACGGUUCUCCUUUGAUdTdT

Antisense strand

dTdTUCCUGCCAAGAGGAAACUA

Chemical modifications

RNA modifications

Human LOX-1 siRNA: 5′-AGGACGGUUCUCCUUUGAU dTdT-3′ (and complement). Mouse LOX-1 siRNA: 5′-GUGGCCAGUUACUACAAAU dTdT-3′ (and complement). Controls: siNonsense; Cy3/Cy5 labeled on 5′ sense strand for uptake/imaging.; 5'-AGGACGGUUCUCCUUUGAU; 5'-GUGGCCAGUUACUACAAAU; 5' sense; dTdT; Cy3; Cy5; dye/fluorescent label