siRNA

Linked experiment: EXP001799

Linked peptide: GALA (fusogenic CPP) — G-NP

Linked paper: Co-delivery of EGFR and BRD4 siRNA by cell-penetrating peptides-modified redox-responsive complex in triple negative breast cancer cells (2021)

RNA overview

RNA Type: siRNA
Payload/target: siEGFR + siBRD4 mixture
Target gene/transcript: EGFR; BRD4

Sequence information

Sequence reported in source article: yes
RNA sequence: siEGFR1/2/3 + siBRD41/2/3; 1:1 siEGFR:siBRD4 mixture
Sense strand: GUCCGCAAGUGUAAGAAGUTT; GCAACAUGUCGAUGGACUUTT; GGAGAUAAGUGAUGGAGAUTT; GCGUUUCCACGGUACCAAATT; CCGUGAUGCUCAGGAGUUUTT; GGAAACCUCAAGCUGAGAATT
Antisense strand: ACUUCUUACACUUGCGGACT; AAGUCCAUCGACAUGUUGCTT; AUCUCCAUCACUUAUCUCCTT; UUUGGUACCGUGGAAACGCTT; AAACUCCUGAGCAUCACGGTT; UUCUCAGCUUGAGGUUUCCTT

Chemical modifications

RNA modifications: siEGFR1/2/3 and siBRD41/2/3 sequences were tested; core experiments used siEGFR + siBRD4 (1:1 mixture). Uptake studies used FAM-labeled negative control siRNA (FAM-siRNA).; 3' TT overhang