siRNA

Linked experiment: EXP001802

Linked peptide: GALA + CREKA (dual CPP) — GC-NP/siBRD4

Linked paper: Co-delivery of EGFR and BRD4 siRNA by cell-penetrating peptides-modified redox-responsive complex in triple negative breast cancer cells (2021)

RNA overview

RNA Type: siRNA
Payload/target: siBRD41/2/3
Target gene/transcript: BRD4

Sequence information

Sequence reported in source article: yes
RNA sequence: siBRD41/2/3 tested
Sense strand: GCGUUUCCACGGUACCAAATT; CCGUGAUGCUCAGGAGUUUTT; GGAAACCUCAAGCUGAGAATT
Antisense strand: UUUGGUACCGUGGAAACGCTT; AAACUCCUGAGCAUCACGGTT; UUCUCAGCUUGAGGUUUCCTT

Chemical modifications

RNA modifications: siEGFR1/2/3 and siBRD41/2/3 sequences were tested; core experiments used siEGFR + siBRD4 (1:1 mixture). Uptake studies used FAM-labeled negative control siRNA (FAM-siRNA).; 3' TT overhang