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siRNA

Linked experiment: EXP001810

Linked peptide: PLGA-PEG-POD / siRNA polyplex

Linked paper: Effective In Vivo Topical Delivery of siRNA and Gene Silencing in Intact Corneal Epithelium Using a Modified Cell-Penetrating Peptide (2019)

RNA overview

RNA Type

siRNA

Payload/target

Uptake: Fluorescent siGLO (GE Dharmacon), green or red labeled (non-targeting). Functional test: luciferase-targeting siLuc vs NSC4 control.

Target gene/transcript

luciferase; siLuc vs NSC4 control

Sequence information

Sequence reported in source article

yes

RNA sequence

sense:CGACAAGCCUGGCGCAGUAUU

Sense strand

CGACAAGCCUGGCGCAGUAUU

Antisense strand

Chemical modifications

RNA modifications

siLuc: 5′-CGACAAGCCUGGCGCAGUAUU-3′ (dTdT overhang at 3′ in both strands); NSC4: 5′-UAGCGACUAAACACAUCAAUU-3′ (dTdT overhang at 3′ in both strands); siGLO fluorescent label.