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gRNA (CRISPR guide RNA) in Cas9 RNP

Linked experiment: EXP001805

Linked peptide: PT24 (oleic aldehyde hydrazone amphiphile from peptide P)

Linked paper: Peptide/Cas9 nanostructures for ribonucleoprotein cell membrane transport and gene edition (2017)

RNA fields

RNA Type

gRNA (CRISPR guide RNA) in Cas9 RNP

RNA Payload Or Target

HPRT1 (gRNA targeting HPRT1 for indel formation); also EGFP editing shown in DF1-EGFP

RNA Modifications

gRNA labelled with ATTO550 in complexation/CLSM assays (for visualization); otherwise unmodified gRNA used for editing