RGD-targeted Au PENP / Bcl-2 siRNA polyplex
Sequence: Arg-Gly-Asp (RGD)
| Experiment Id | EXP002419 |
|---|---|
| Paper | Multifunctional PEI-entrapped gold nanoparticles enable efficient delivery of therapeutic siRNA into |
| Peptide | RGD-targeted Au PENP / Bcl-2 siRNA polyplex |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | not separately reported; vector/siRNA polyplex prepared by N/P ratio |
| Rna Concentration | 20 µg siRNA per mouse |
| Mixing Ratio | N/P ratio 10:1 for functional in vitro and in vivo assays |
| Formulation Format | Targeted nanoparticle/siRNA polyplex |
| Formulation Components | Bcl-2 siRNA complexed with {(Au0)25-PEI-(PEG-RGD)10-mPEG10} PENPs; PEGylated PEI, PEGylated RGD, mPEG, entrapped Au NPs. |
| Size Nm | 24.20 |
| Zeta Mv | 7.00 |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | U87MG xenografted tumor cells |
| Animal Model | BALB/c nude mice bearing U87MG xenograft tumors (~300 mm3) |
| Administration Route | Intratumoral injection |
| Output Type | In vivo Bcl-2 protein knockdown by western blot; tumor-cell apoptosis by TUNEL assay |
| Output Value | Targeted Au PENP/Bcl-2 siRNA reduced Bcl-2 protein expression to 25.5% of control in vivo; TUNEL-positive apoptosis rate 20.57%. |
| Output Units | |
| Output Notes | Mice were sacrificed on day 4 after intratumoral injection; targeted RGD Au PENP/siRNA produced stronger knockdown and apoptosis than PEI/siRNA, non-targeted Au PENP/siRNA, free siRNA, or saline. |
| Toxicity Notes | H&E staining after targeted Au PENP/siRNA treatment for 7 and 14 days showed no significant abnormality or damage in heart, liver, spleen, lung, or kidney. |
| Curation Notes |