Synergistic breast cancer therapy with RGD-decorated liposomes co-delivering mir-34a and cisplatin (2024)
RGD-decorated liposome / dipalmitoyl-GRGDSPA
Sequence: GRGDSPA
| Experiment Id | EXP002453 |
|---|---|
| Paper | Synergistic breast cancer therapy with RGD-decorated liposomes co-delivering mir-34a and cisplatin |
| Peptide | RGD-decorated liposome / dipalmitoyl-GRGDSPA |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | not separately specified; dipalmitoyl-GRGDSPA included in lipid mixture at DSPC:cholesterol:dipalmitoyl-GRGDSPA 7:3:1 mM |
| Rna Concentration | In vitro assays used 10 nM miR-34a for apoptosis/gene-expression experiments; MTT included 5-10 nM miR-34a with cisplatin held at 16 ug/mL in combination assays |
| Mixing Ratio | Lipid composition DSPC:cholesterol:dipalmitoyl-GRGDSPA = 7:3:1 mM; protamine/miR-34a complex formed before liposome hydration |
| Formulation Format | RGD-modified liposome co-delivering cisplatin and protamine-complexed miR-34a |
| Formulation Components | DSPC, cholesterol, dipalmitoyl-GRGDSPA, protamine, miR-34a, cisplatin |
| Size Nm | 213.90 |
| Zeta Mv | -10.70 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MCF-7 human breast cancer cells |
| Animal Model | |
| Administration Route | cell culture exposure to liposomal formulations |
| Output Type | cell viability; apoptosis; apoptosis-related gene expression |
| Output Value | miR34a-Cis-Lip-RGD IC50 3.90 +/- 0.23 nM at 24 h; apoptosis 76.24%; increased Bax expression and decreased Bcl-2 expression relative to controls |
| Output Units | |
| Output Notes | Functional in vitro RNA-associated antitumor effect shown, but in vitro activity does not qualify as delivery_success_class = 1. |
| Toxicity Notes | Hemolysis assay: cisplatin caused RBC lysis, while liposomal encapsulation significantly decreased lysis compared with free cisplatin. |
| Curation Notes |