Sequence: cyclo(RGDfK); main text reports cyclo-RGDfK/cRGD peptide, with N-terminal cysteine used for conjugation
| Experiment Id | EXP002504 |
|---|---|
| Paper | Multifunctional polyion complex micelle featuring enhanced stability, targetability, and endosome es |
| Peptide | cRGD-PEG-PAsp(TEP)-Chol |
| Delivery Success Class | no |
| In Vivo Flag | yes |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 25 µg siRNA/mouse for systemic tumor accumulation and intravital imaging studies |
| Mixing Ratio | N/P = 3 |
| Formulation Format | targeted/stabilized polyion complex micelle |
| Formulation Components | cRGD-PEG-PAsp(TEP)-Chol block copolymer complexed with fluorescent siLuc; 5% glucose adjusted solution |
| Size Nm | -50.00 |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo biodistribution/tumor accumulation |
| Cell Lines Or Primary Cells | A549 human lung carcinoma xenograft |
| Animal Model | BALB/c nude mice bearing subcutaneous A549 tumors |
| Administration Route | intravenous tail vein |
| Output Type | tumor accumulation / intravital confocal imaging |
| Output Value | targeted/stabilized cRGD(+)/Chol(+) micelle showed higher A549 tumor accumulation than naked siRNA and single-function controls |
| Output Units | |
| Output Notes | This row captures delivery/accumulation evidence for the final cRGD- and Chol-containing carrier using fluorescent siLuc, not functional reporter knockdown. |
| Toxicity Notes | No liver toxicity was observed in later systemic micelle study based on ALT/AST. |
| Curation Notes |