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EXP000534

Paper

Efficient systemic CNS delivery of a therapeutic antisense oligonucleotide with a blood-brain barrier-penetrating ApoE-derived peptide (2024)

Peptide

BPP8

Sequence: TPA-mPEG-rllrkrlkrlhsalrvrl

RNA

PMO

All experiment fields

Experiment Id EXP000534
Paper Efficient systemic CNS delivery of a therapeutic antisense oligonucleotide with a blood-brain barrie
Peptide BPP8
Delivery Success Class yes
In Vivo Flag yes
Uptake Confirmed no
Label Confidence high
In Vitro Functional Effect
Endosomal Escape Evidence
Peptide Concentration
Rna Concentration 8 mg/kg (BPP8); 4 mg/kg (BPP9); 10 mg/kg (BPP12)
Mixing Ratio
Formulation Format Systemic peptide–PMO conjugate
Formulation Components ApoE- or THR-derived BPP–PMO conjugate
Size Nm
Zeta Mv
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model SMN2+/- transgenic mice (adult)
Administration Route IV (tail vein)
Output Type In vivo splice correction; biodistribution imaging
Output Value BPP8 significantly increased SMN2 in brain and spinal cord; others ineffective
Output Units
Output Notes Only BPP8 demonstrated effective BBB penetration and CNS antisense activity
Toxicity Notes No hepatotoxicity or immune activation for BPP8
Curation Notes