Sequence: CGRRMKWKK-[photo]-EEEERRRR
| Experiment Id | EXP000781 |
|---|---|
| Paper | Polymer nanoparticles modified with photo- and pH-dual-responsive polypeptides for enhanced and targ |
| Peptide | PPP (activated) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | PLGA nanoparticles (w/o/w double emulsion) PEGylated with DSPE-PEG2000; surface ligand DSPE-PEG2000-peptide; siRNA encapsulated as siRNA–spermidine complex |
| Formulation Components | PLGA (50/50); DSPE-PEG2000; PVA; siRNA–spermidine complex |
| Size Nm | 116.27 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MCF-7 (human breast adenocarcinoma) |
| Animal Model | |
| Administration Route | |
| Output Type | EGFR gene knockdown (qRT-PCR / western blot) |
| Output Value | |
| Output Units | |
| Output Notes | Uptake measured by flow cytometry and confocal microscopy using FAM-siRNA (75 nM for flow, 225 nM for confocal). PPP-NPs required both NIR pretreatment (740 nm, 50 J/cm^2, 30 min) and acidic medium (pH 6.0) for high uptake. Endosomal/lysosomal escape shown by LysoTracker colocalization at 0.5 h and cytosolic siRNA at 2 h after transfection (after 4 h incubation). EGFR-siRNA delivered by PPP-NPs (NIR, pH 6.0) reduced EGFR mRNA/protein and induced apoptosis. Encapsulation efficiency ~87% for all NP formulations. |
| Toxicity Notes | |
| Curation Notes |