Sequence: GLWRALWRLLRSLWRLLWKA
siRNA (multimeric antisense/guide strands annealed to PNA sense)
| Experiment Id | EXP001148 |
|---|---|
| Paper | HER2 RNA Aptamer- and Cell Penetrating Peptide-Mediated Delivery of Multimeric Antisense Strands of |
| Peptide | CADY |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 500 nM (monomeric antisense equivalents) |
| Rna Concentration | 500 nM (monomeric antisense equivalents) |
| Mixing Ratio | Equimolar RNA:PNA in annealing buffer (Tris/NaCl); CPP is covalently linked to PNA |
| Formulation Format | CPP–PNA-annealed multimeric siRNA (hybridized multimeric antisense + CPP-PNA sense) |
| Formulation Components | Multimeric antisense (HS-UGCGCUCCUGGACGUAGCCUU-SH) polymer + CADY-8mer PNA (Ac-GGAGCGCA-GLWRALWRLLRSLWRLLWKA) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa; MCF-7 (HER2-negative); PC3 (cell-type comparison; eGFP-expressing) |
| Animal Model | |
| Administration Route | |
| Output Type | Functional gene silencing (cell-type comparison of eGFP fluorescence decrease) |
| Output Value | Gene silencing observed across multiple cell types; described as non-target-specific vs HER2 aptamer system |
| Output Units | |
| Output Notes | Authors report CPP-PNA-annealed multimeric siRNA lacks cell-type specificity (unlike HER2 aptamer system). |
| Toxicity Notes | |
| Curation Notes |