siRNA (multimeric antisense/guide strands annealed to PNA sense)
RNA Payload Or Target
eGFP (enhanced GFP) in eGFP-expressing cells
RNA Modifications
Antisense strand is di-thiolated at 5′ and 3′ ends and polymerized into multimeric antisense strands (>150–500-mer by gel, SI); annealed to 8-mer PNA complementary to antisense