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EXP001175

Paper

Macrophage membrane- and cRGD-functionalized thermosensitive liposomes combined with CPP to realize precise siRNA delivery into tumor cells (2022)

Peptide

CPP

Sequence: CKRRMKWKKC

RNA

siRNA

All experiment fields

Experiment Id EXP001175
Paper Macrophage membrane- and cRGD-functionalized thermosensitive liposomes combined with CPP to realize
Peptide CPP
Delivery Success Class no
In Vivo Flag yes
Uptake Confirmed no
Label Confidence high
In Vitro Functional Effect
Endosomal Escape Evidence
Peptide Concentration Covalent CPP-siRNA conjugate
Rna Concentration 1.2 mg/kg (siRNA equivalent)
Mixing Ratio 1:1 (covalent conjugate)
Formulation Format Free C-siRNA (CPP–siRNA conjugate) (no carrier)
Formulation Components CPP disulfide-linked to thiol-modified Bcl-2 siRNA (C-siRNA); administered IV
Size Nm
Zeta Mv
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model Male nude mice bearing subcutaneous HepG2 tumor xenografts
Administration Route Intravenous (tail vein); tumor region heated to 42°C for 30 min after injection (except U group).
Output Type Tumor growth inhibition (in vivo)
Output Value No tumor inhibition vs control; non-specific distribution and fast elimination
Output Units
Output Notes In vivo distribution resembled free siRNA (systemic, bladder/kidney clearance); no evident tumor targeting or tumor suppression.
Toxicity Notes
Curation Notes