Sequence: AGYLLGKINLKALAALAKKIL
PNA (antisense; fluorescein-labeled) + NLS peptide tail
| Experiment Id | EXP001315 |
|---|---|
| Paper | Application of Cell-Penetrating Peptides (CPP) to Enhance the Efficacy of Antisense Peptide Nucleic |
| Peptide | TP10 (Transportan 10) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | Example conditions: 80 pmol PNA + 60 pmol TP10 per well (3 h); plate reader optimization also reports 6.7 pmol PNA + 5.1 pmol TP10. |
| Formulation Format | non-covalent CPP–PNA complex (co-incubation) |
| Formulation Components | TP10 + Fluo-PCSK9-PPT-EX1-Ahx-NLS mixed in Opti-MEM, incubated 30 min at RT; added to HeLa/HEK293T cells for 3 h; wash with PBS before readouts. |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa (human cervical cancer) |
| Animal Model | |
| Administration Route | |
| Output Type | uptake / internalization |
| Output Value | Increased cellular fluorescence (microscopy + plate reader); higher fluoPNA signal with TP10 co-incubation vs PNA alone; HPLC AUC higher for PNA+TP10 extracts. |
| Output Units | |
| Output Notes | Readouts: fluorescence microscopy (Fig.1), plate reader fluorescence intensity (Fig.2), Coomassie-stained PVDF after electrophoresis as proxy for fluoPNA level (Fig.3), and HPLC fluorescence detection in cell extracts (Fig.4). |
| Toxicity Notes | |
| Curation Notes |