N/A (DNA tetrahedron vector; no peptide carrier)
Sequence: CKRRMKWKK
PNA (antisense oligonucleotide)
| Experiment Id | EXP001428 |
|---|---|
| Paper | Tetrahedral DNA Nanoparticle Vector for Intracellular Delivery of Targeted Peptide Nucleic Acid Anti |
| Peptide | N/A (DNA tetrahedron vector; no peptide carrier) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 0–40 µM PNA4 (as part of PT01); tested with cefotaxime (CTX) 16 mg/L in growth assay |
| Mixing Ratio | Equimolar ssDNA component strands + PNA4 during annealing (assembly buffer: Tris/MgCl2; heat 95°C then rapid cool) |
| Formulation Format | DNA tetrahedron nanoparticle carrying antisense PNA (PT01) |
| Formulation Components | PT01-S1 + PT01-S2 + PT01-S3 + PT01-S4 (ssDNA) + PNA4 (13-mer) self-assembled tetrahedron |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Escherichia coli field isolate LREC461 (human origin), blaCTX-M-3 plasmid; cefotaxime-resistant |
| Animal Model | |
| Administration Route | |
| Output Type | Antibiotic potentiation (MIC to cefotaxime; growth inhibition threshold O.D.600<0.1 at 18 h) |
| Output Value | 16 |
| Output Units | |
| Output Notes | Dose-dependent CTX potentiation observed when PNA4 was carried by PT01; at 40 µM PT01 reduced CTX MIC from 35 mg/L (baseline) to 16 mg/L. PT01 alone showed no growth inhibition. |
| Toxicity Notes | DNA tetrahedron PT01 alone (10–30 µM) showed no significant growth inhibitory effect. |
| Curation Notes |