CPP5-ABE8e (covalent CPP fusion)
Sequence: KLPVM
| Experiment Id | EXP001438 |
|---|---|
| Paper | Safer and efficient base editing and prime editing via ribonucleoproteins delivered through optimize |
| Peptide | CPP5-ABE8e (covalent CPP fusion) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | medium |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | Protein concentration tested up to ~2 µM (ABE8e RNP equivalents) in reporter assay |
| Rna Concentration | Guide RNA present in RNP; concentrations tested 0–2 µM RNP |
| Mixing Ratio | Delivered as pre-assembled RNP in OptiMEM with 2% or 10% (w/v) sucrose |
| Formulation Format | CPP-fused ABE8e RNP (direct delivery, no LNP) |
| Formulation Components | CPP-fused ABE8e protein + sgRNA (rd12-A6) (+ sucrose as indicated) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | NIH/3T3-derived rd12 fluorescent reporter cells (mCherry–rd12–eGFP reporter) |
| Animal Model | |
| Administration Route | |
| Output Type | Base-editing functional readout: reporter conversion (mCherry+eGFP by flow cytometry) |
| Output Value | |
| Output Units | |
| Output Notes | CPP fusion did not improve ABE8e RNP delivery efficiency versus unmodified ABE8e under their tested in vitro conditions. |
| Toxicity Notes | |
| Curation Notes |