CTX-R10 (chlorotoxin + deca-arginine)
Sequence: CTX (not specified) ; RRRRRRRRRR
| Experiment Id | EXP001450 |
|---|---|
| Paper | Iron oxide nanoparticles decorated with functional peptides for a targeted siRNA delivery to glioma |
| Peptide | CTX-R10 (chlorotoxin + deca-arginine) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | medium |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | NP-CTX-R10 used at 20 µg/mL (Fe) for uptake assays |
| Rna Concentration | Not specified in SI for SF763 uptake experiment (NP fluorescence used for uptake quantification) |
| Mixing Ratio | |
| Formulation Format | Peptide-decorated iron oxide nanoparticles (NP-CTX) for targeting uptake assays (no siRNA specified in SF763 uptake method) |
| Formulation Components | NP coated with CCP-PEI polymer + CTX targeting peptide (Cy5-labeled for flow cytometry uptake). |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | SF763 human glioblastoma cells |
| Animal Model | |
| Administration Route | |
| Output Type | Uptake/internalization (Cy5 fluorescence by flow cytometry, 4 h) |
| Output Value | |
| Output Units | |
| Output Notes | Supplementary Methods describe NP vs NP-CTX uptake analysis by flow cytometry in SF763 cells (same protocol as C6). Functional siRNA effect in SF763 is not shown in this SI file. |
| Toxicity Notes | |
| Curation Notes |