Sequence: Ac-Tyr-Lys-Leu-Dpg-Leu-Lys-Leu-Dpg-Leu-NH2
| Experiment Id | EXP001532 |
|---|---|
| Paper | An Amphipathic Structure of a Dipropylglycine-Containing Helical Peptide with Sufficient Length Enab |
| Peptide | Pep5 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | 8.4 µM (for N/P=4 with 100 nM siRNA) |
| Rna Concentration | 100 nM (Luc or Scr siRNA); 100 nM Alexa488-siRNA for uptake assay |
| Mixing Ratio | N/P = 4 (amino/guanidino groups : siRNA phosphates) |
| Formulation Format | electrostatic peptide/siRNA complexes (CPP nanoparticles) |
| Formulation Components | Pep5 + siRNA (Luc or Scr) / Alexa488-siRNA (uptake) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Huh-7-Luc (luciferase-expressing human hepatoma cells; 10% FBS media) |
| Animal Model | |
| Administration Route | |
| Output Type | Uptake (flow cytometry + fluorescence microscopy) and RNAi (luciferase assay, 24 h) |
| Output Value | Luciferase signal decreased for both Luc and Scr siRNA with increasing N/P (toxicity-dominated; not specific knockdown). |
| Output Units | |
| Output Notes | Very low Alexa488-siRNA uptake (flow cytometry low; almost no microscopy signal). RNAi measured in 10% FBS at 24 h; Lipofectamine 3000 ineffective under these conditions. |
| Toxicity Notes | Severe cytotoxicity at N/P=4 and N/P=8 (24 h); luciferase reduced regardless of siRNA sequence. |
| Curation Notes |