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siRNA

Linked experiment: EXP001532

Linked peptide: Pep5

Linked paper: An Amphipathic Structure of a Dipropylglycine-Containing Helical Peptide with Sufficient Length Enables Safe and Effective Intracellular siRNA Delivery (2023)

RNA fields

RNA Type

siRNA

RNA Payload Or Target

Firefly GL3 luciferase siRNA (Luc siRNA); sequences: sense 5'-CUUACGCUGAGUACUUCGAdTdT-3', antisense 5'-UCG AAG UAC UCA GCG UAA GdTdT-3' (dTdT overhangs). Control: Scrambled control siRNA (Scr siRNA); sequences: sense 5'-UUCUCCGAACGUGUCACGUdTdT-3', antisense 5'-ACGUGACACGUUCGGAGAAdTdT-3'.

RNA Modifications

dTdT overhangs; Alexa488 label on antisense strand used for uptake experiments (separate assay).