Novel lipidated sorbitol-based molecular transporters for non-viral gene delivery (2009)
1b (C24 lipidated sorbitol octaguanidine)
Sequence: N/A (non-peptidic guanidine-based molecular transporter)
| Experiment Id | EXP001707 |
|---|---|
| Paper | Novel lipidated sorbitol-based molecular transporters for non-viral gene delivery |
| Peptide | 1b (C24 lipidated sorbitol octaguanidine) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | STR-R8 5 mol% on surface; transporter amount set by N/P=3 |
| Rna Concentration | 0.4 µg siRNA per well |
| Mixing Ratio | siRNA condensed at N/P=3; STR-R8 5 mol% (surface); 0.4 µg siRNA per well |
| Formulation Format | siRNA MEND: condensed siRNA core + lipid envelope, surface-modified with STR-R8 |
| Formulation Components | Core: siRNA condensed with transporter at N/P=3; Envelope: DOPE/PA (7:2 molar); Surface ligand: STR-R8 (5 mol% of total lipid) |
| Size Nm | 295.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa-luciferase stable cells |
| Animal Model | |
| Administration Route | |
| Output Type | in vitro gene knockdown (luciferase silencing) |
| Output Value | Robust luciferase knockdown for all tested transporter-based siRNA MENDs; 1a-core gave the highest silencing effect (lowest % luciferase relative to anti-GFP control). |
| Output Units | |
| Output Notes | Silencing calculated vs cells treated with control anti-GFP siRNA; luciferase measured 24 h post-transfection. |
| Toxicity Notes | |
| Curation Notes |