GALA + CREKA (dual CPP) — GC-NP
Sequence: GALA: nan; CREKA: CREKA
| Experiment Id | EXP001945 |
|---|---|
| Paper | Co-delivery of EGFR and BRD4 siRNA by cell-penetrating peptides-modified redox-responsive complex in |
| Peptide | GALA + CREKA (dual CPP) — GC-NP |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | N/P = 30 (optimal) |
| Formulation Format | Electrostatic polyplex (PEG-SS-PEI/siRNA) with CPP (GALA and/or CREKA) modification |
| Formulation Components | GC-NP: GALA-PEG-SS-PEI + CREKA-PEG-SS-PEI complexed with siRNA (siEGFR:siBRD4 = 1:1) |
| Size Nm | 70.00 |
| Zeta Mv | 8.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MDA-MB-231 (TNBC) for uptake/gene silencing/phenotype; HUVEC for biocompatibility |
| Animal Model | |
| Administration Route | |
| Output Type | In vitro functional RNA effect: qRT-PCR + western blot (EGFR/PI3K/Akt/BRD4/c-Myc) and phenotypic assays |
| Output Value | Synergistic inhibition of EGFR/PI3K/Akt and BRD4/c-Myc signaling; reduced proliferation (CCK-8), invasion (Transwell), and migration (wound healing) vs single-siRNA groups |
| Output Units | |
| Output Notes | Uptake quantified by confocal microscopy + flow cytometry with FAM-siRNA; lysosomal escape demonstrated by time-dependent decrease in LysoTracker colocalization (Pearson Rr decreases 0.76→0.52). |
| Toxicity Notes | |
| Curation Notes |