Sequence: CGGGARKKAAKAARKKAAKAARKKAAKAARKKAAKA
| Experiment Id | EXP001963 |
|---|---|
| Paper | Effective In Vivo Topical Delivery of siRNA and Gene Silencing in Intact Corneal Epithelium Using a |
| Peptide | POD / siRNA polyplex |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | 35:1 (POD:siRNA molar ratio) in PBS; 1 µM siRNA used in most in vitro assays. |
| Formulation Format | Noncovalent CPP–siRNA polyplex |
| Formulation Components | POD complexed with siRNA in PBS (35:1). |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HCE-S human corneal epithelial cells |
| Animal Model | |
| Administration Route | |
| Output Type | In vitro uptake (fluorescence microscopy); in vitro luciferase knockdown (dual luciferase assay) |
| Output Value | Uptake observed as punctate perinuclear cytoplasmic fluorescence; no significant luciferase knockdown without endosomal disruptor. |
| Output Units | |
| Output Notes | Authors infer endosomal entrapment because uptake without knockdown; used chloroquine to test escape hypothesis. |
| Toxicity Notes | MTT viability generally ~75–100% at 17.5–35 µM (drops to ~55% at 70 µM for Chol-/Palm-/POD). |
| Curation Notes |