Chol-POD + chloroquine / siRNA polyplex
Sequence: CGGGARKKAAKAARKKAAKAARKKAAKAARKKAAKA
| Experiment Id | EXP001964 |
|---|---|
| Paper | Effective In Vivo Topical Delivery of siRNA and Gene Silencing in Intact Corneal Epithelium Using a |
| Peptide | Chol-POD + chloroquine / siRNA polyplex |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | 35:1 (POD:siRNA) with 1 µM siRNA; chloroquine 30 µM added 1 h before transfection. |
| Formulation Format | CPP–siRNA polyplex + small-molecule endosomal disruptor |
| Formulation Components | Chol-POD polyplexed with siRNA (35:1) in PBS; free chloroquine added (30 µM). |
| Size Nm | 150.80 |
| Zeta Mv | 15.60 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HCE-S human corneal epithelial cells |
| Animal Model | |
| Administration Route | |
| Output Type | In vitro functional RNA effect (luciferase knockdown) + endosomal escape proxy (diffuse cytoplasmic siGLO pattern) |
| Output Value | Significant luciferase knockdown observed only when chloroquine was used with the polyplexes; siGLO fluorescence becomes more diffuse vs punctate. |
| Output Units | |
| Output Notes | Used to demonstrate endosomal entrapment barrier and that escape enables RNAi activity. |
| Toxicity Notes | Chloroquine noted as toxic for in vivo use; therefore replaced by covalently attached analog for in vivo. |
| Curation Notes |