Sequence: NH2-Lys-A*A*C*A*T*C*A*G*T*C*T*G*A*T*A*A*G*C*T*A*-Lys-CONH2
| Experiment Id | EXP002100 |
|---|---|
| Paper | Thyclotides, tetrahydrofuran-modified peptide nucleic acids that efficiently penetrate cells and inh |
| Peptide | Lys-thyclotide (anti-miR-21) |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | 1 nM–5 µM |
| Rna Concentration | endogenous miRNA |
| Mixing Ratio | N/A (antisense binding) |
| Formulation Format | single-molecule antisense thyclotide |
| Formulation Components | thyclotide only |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | HepG2; SKHEP1; RKO; MCF7 |
| Cell Lines Or Primary Cells | miRNA inhibition / gene derepression |
| Animal Model | miR-21 functional inhibition; PTEN, Cdc25a, KRIT1 upregulation |
| Administration Route | Efficient cytosolic delivery without CPPs; strong miR-21 inhibition at nanomolar concentrations |
| Output Type | 0 |
| Output Value | 0 |
| Output Units | |
| Output Notes | 1 |
| Toxicity Notes | high |
| Curation Notes |