3TAT-DRBD (within STD fusion protein)

Peptide ID: PEPTIDE001149
Raw sequence: GRKKRRQRRRGSHGRKKRRQRRRGSHGRKKRRQRRRGSHAGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKA
Clean backbone: GRKKRRQRRRGSHGRKKRRQRRRGSHGRKKRRQRRRGSHAGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKA
Tokenized backbone: G-R-K-K-R-R-Q-R-R-R-G-S-H-G-R-K-K-R-R-Q-R-R-R-G-S-H-G-R-K-K-R-R-Q-R-R-R-G-S-H-A-G-D-L-S-A-G-F-F-M-E-E-L-N-T-Y-R-Q-K-Q-G-V-V-L-K-Y-Q-E-L-P-N-S-G-P-P-H-D-R-R-F-T-F-Q-V-I-I-D-G-R-E-F-P-E-G-E-G-R-S-K-K-E-A-K-N-A-A-A-K-L-A-V-E-I-L-N-K-E-K-K-A
Sequence engineering
Stereochemistry
Modifications: Delivery backbone is a fusion protein 'STD' (streptavidin + 3×TAT + DRBD). 3×TAT repeats (10 aa each) connected by GSH linkers; DRBD is motif 1 of human PKR (residues 9–78). Streptavidin binds biotinylated A10 aptamer; DRBD binds siRNA to mask charge.
Sequence length: 119
Noncanonical residues

Experiments