rd12-targeting sgRNA (ABE) or epegRNA (PE) for Rpe65 rd12 mutation correction (R44X); non-targeting guides used as controls in some experiments.
RNA Modifications
End-modified guide RNAs: 2′-O-methyl on first 3 and last 3 nucleotides; first 3 and last 3 phosphodiester bonds replaced with phosphorothioates (as described in Methods).