Uptake: Fluorescent siGLO (GE Dharmacon), green or red labeled (non-targeting). Functional test: luciferase-targeting siLuc vs NSC4 control.
RNA Modifications
siLuc: 5′-CGACAAGCCUGGCGCAGUAUU-3′ (dTdT overhang at 3′ in both strands); NSC4: 5′-UAGCGACUAAACACAUCAAUU-3′ (dTdT overhang at 3′ in both strands); siGLO fluorescent label.