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siRNA (siGLO; siLuc; NSC4)

Linked experiment: EXP001962

Linked peptide: PLGA-PEG-POD / siRNA polyplex

Linked paper: Effective In Vivo Topical Delivery of siRNA and Gene Silencing in Intact Corneal Epithelium Using a Modified Cell-Penetrating Peptide (2019)

RNA fields

RNA Type
siRNA (siGLO; siLuc; NSC4)
RNA Payload Or Target
Uptake: Fluorescent siGLO (GE Dharmacon), green or red labeled (non-targeting). Functional test: luciferase-targeting siLuc vs NSC4 control.
RNA Modifications
siLuc: 5′-CGACAAGCCUGGCGCAGUAUU-3′ (dTdT overhang at 3′ in both strands); NSC4: 5′-UAGCGACUAAACACAUCAAUU-3′ (dTdT overhang at 3′ in both strands); siGLO fluorescent label.