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siRNA (siLuc vs NSC4; siGLO)

Linked experiment: EXP001966

Linked peptide: PLGA-PEG-POD + chloroquine / siRNA polyplex

Linked paper: Effective In Vivo Topical Delivery of siRNA and Gene Silencing in Intact Corneal Epithelium Using a Modified Cell-Penetrating Peptide (2019)

RNA fields

RNA Type
siRNA (siLuc vs NSC4; siGLO)
RNA Payload Or Target
Luciferase knockdown (siLuc) with chloroquine-assisted endosomal escape; uptake tracked with siGLO.
RNA Modifications
siLuc: 5′-CGACAAGCCUGGCGCAGUAUU-3′ (dTdT overhang at 3′ in both strands); NSC4: 5′-UAGCGACUAAACACAUCAAUU-3′ (dTdT overhang at 3′ in both strands); siGLO fluorescent label.