Back to browse

siRNA (siLuc vs NSC4; siGLO)

Linked experiment: EXP001968

Linked peptide: QN-Palm-POD / siRNA polyplex

Linked paper: Effective In Vivo Topical Delivery of siRNA and Gene Silencing in Intact Corneal Epithelium Using a Modified Cell-Penetrating Peptide (2019)

RNA fields

RNA Type

siRNA (siLuc vs NSC4; siGLO)

RNA Payload Or Target

Luciferase knockdown in HCE-S cells (siLuc) without free chloroquine; uptake tracked with siGLO.

RNA Modifications

siLuc: 5′-CGACAAGCCUGGCGCAGUAUU-3′ (dTdT overhang at 3′ in both strands); NSC4: 5′-UAGCGACUAAACACAUCAAUU-3′ (dTdT overhang at 3′ in both strands); siGLO fluorescent label.