GALA + CREKA (dual CPP) — GC-NP/siBRD4
Sequence: GALA: nan; CREKA: CREKA
| Experiment Id | EXP001949 |
|---|---|
| Paper | Co-delivery of EGFR and BRD4 siRNA by cell-penetrating peptides-modified redox-responsive complex in |
| Peptide | GALA + CREKA (dual CPP) — GC-NP/siBRD4 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | N/P = 30 (optimal) |
| Formulation Format | Electrostatic polyplex (PEG-SS-PEI/siRNA) with CPP (GALA and/or CREKA) modification |
| Formulation Components | GC-NP/siBRD4: dual CPP polymer complex with siBRD4 (single payload) |
| Size Nm | 70.00 |
| Zeta Mv | 8.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MDA-MB-231 (TNBC) for uptake/gene silencing/phenotype; HUVEC for biocompatibility |
| Animal Model | |
| Administration Route | |
| Output Type | In vitro functional RNA effect: BRD4/c-Myc pathway suppression; reduced invasion/migration vs control |
| Output Value | siBRD4 alone decreases BRD4 and downstream c-Myc; less than dual-siRNA synergy on PI3K/Akt+c-Myc |
| Output Units | |
| Output Notes | Used to compare against co-silencing; same delivery vehicle chemistry. |
| Toxicity Notes | |
| Curation Notes |