Back to browse

EXP002354

Paper

Systemic delivery of a peptide-linked morpholino oligonucleotide neutralizes mutant RNA toxicity in a mouse model of myotonic dystrophy (2013)

Peptide

Peptide B

Sequence: AcRXRRBRRXRRBRXB-OH

RNA

antisense morpholino (PPMO)

All experiment fields

Experiment Id EXP002354
Paper Systemic delivery of a peptide-linked morpholino oligonucleotide neutralizes mutant RNA toxicity in
Peptide Peptide B
Delivery Success Class yes
In Vivo Flag yes
Uptake Confirmed no
Label Confidence high
In Vitro Functional Effect
Endosomal Escape Evidence
Peptide Concentration
Rna Concentration
Mixing Ratio
Formulation Format peptide–morpholino conjugate (PPMO)
Formulation Components Peptide B-CAG25 PPMO
Size Nm
Zeta Mv
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model HSALR transgenic mouse model of myotonic dystrophy type 1
Administration Route intravenous (tail vein), weekly ×6
Output Type phenotypic rescue and molecular correction
Output Value Correction of RNA splicing (Serca-1, ClC-1, Titin, Zasp); elimination of myotonia
Output Units
Output Notes Redistribution of Mbnl1 from nuclear foci; normalization of EMG signals
Toxicity Notes No overt toxicity; normal ALT/AST, BUN, creatinine
Curation Notes